Assistant Professor Amy Ham of the Department of Pharmaceutical, Social & Administrative Sciences has published “Proteomic Profiling of Paraffin-Embedded Samples Identifies Metaplasia-Specific and Early-Stage Gastric Cancer Biomarkers” in The American Journal of Pathology.
Early diagnosis and curative resection are the predominant factors associated with increased survival in patients with gastric cancer, yet most gastric cancers are detected in late stage disease. In this paper, the authors analyzed formalin-fixed paraffin embedded (FFPE) stomach tissues of Korean patients from intestinal-type gastric cancer, metaplasia and normal mucosa using proteomic technologies to look at protein expression differences with the goal of finding potential protein biomarkers for the early detection of gastric cancer. Selected proteins were analyzed first using peptide isoelectric focusing and liquid chromatography-tandem mass spectrometry analysis to generate proteomic profiles. Selected proteins were subsequently analyzed by immunostaining in larger tissue array cohorts in an effort to verify the findings. The authors detected 60 proteins up-regulated and 87 proteins down-regulated during the progression from normal mucosa to metaplasia to gastric cancer. These studies led to the identification of two novel markers for stomach metaplasias and gastric cancer prognosis.